eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

JEOL JEM-4000FX TEM

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Initial Alignment

High Voltage Centering Adjustment

1. Set the SPOT SIZE=1, MAG=~100 kX.

    
2. Spread the beam with the BRIGHTNESS knob and center a point of interest on specimen.

NOTE: It is helpful to select a point on the sample with features that have both an X and Y orientation.		 VIEW

3. Activate GATAN CAMERA

    
3.1. IMPORTANT: Spread the illumination with BRIGHTNESS knob. VIEW
3.2. Push the SCREEN button 2 times to raise the screen. VIEW
3.3. In the DigitalMicrograph program within the Camera View panel select Setup > Focus and Exposure Mode > Optimize. VIEW
3.4. Check the Camera Inserted box. VIEW
3.5. Click START view.
3.6. Enter MAG in the menu box, then OK.
4. Focus the image using the OBJ FOCUS knob. VIEW
5. Engage the WOBBLER, HT (on the right main panel). VIEW
6. Engage the DEFLECTOR, BRIT TILT button (on the left main panel). VIEW

7. Using the DEF X,Y knobs and the GATAN CAMERA image, try to eliminate specimen translation. As you turn either (but not both, simultaneously) the X or Y knob, look for both a decrease in the magnitude of the translation and a change in the direction of translation. When this occurs, turn the other knob. Repeat until the translation is eliminated and the image appears to "blink".

    
8. Push the SCREEN button one time to lower the screen. VIEW
9. Disengage the DEFLECTOR, BRIT TILT and the WOBBLER, HT buttons. VIEW
10. Click Stop View in the DigitalMicrograph software. VIEW

11. Uncheck the Camera Inserted box.

    

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