eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

JEOL JEM-4000FX TEM

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Initial Alignment

Gun and Condenser Lens Alignment

1. Resaturate the FILAMENT slowly.

   

2. Set the SPOT SIZE=1, MAG=~50 kX.

   
3. Engage the GUN button (in the right hand drawer). VIEW
4. Focus the illumination to a spot using the BRIGHTNESS knob. VIEW
5. Disengage the X 10 button. VIEW

6. Center the illumination using the GUN SHIFT X, Y knobs in the right drawer.

VIEW
7. Set the SPOT SIZE=6 and focus the illumination to a spot using the BRIGHTNESS knob. VIEW

8. Center the illumination using the SHIFT X, Y knobs on the main panel.

VIEW

9. Repeat steps 2 - 8 until there is no displacement of the illumination with the SPOT SIZE change.

VIEW
10. Disengage the GUN button. VIEW
11. Spread the beam using the BRIGHTNESS knob. VIEW
12. Engage the X 10 button. VIEW

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