eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

JEOL JEM-4000FX TEM

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

FAQs

What are common issues for EDS?

  1. Objective Aperture must be out for EDS use.

  2. Carefully watch the dead time percent on the Revolution software to prevent the system from locking up. Landing the beam on a grid bar will shoot the dead time percent very high and freeze the system. Use a smaller condenser aperture or smaller spot size to lower the dead time.

  3. The film camera will vent if the lever on the cover is turned horizontally. This will shut the beam off. Contact lab personnel for help.

  4. Accelerating voltage is off. Contact lab personnel for help.

  5. The current meter is 0.00. Contact lab personnel for help.

  6. The current meter is reading greater than 10 µA of dark current. You need to decrease the fine bias.

What happens if the beam is lost?

Knob TweakingSTOP! Do not turn or push any controls. Remember what you were doing when the beam was lost. The beam can be easily lost when adjusting the filament image. See Gun Alignment under Initial Alignment in JEOL JEM-4000FX TEM. Contact lab personnel for help.

What should I do if the vacuum is poor?

Contact lab personnel for help.

What if there is no beam on start up?

Lower the magnification to 5000X. Use the specimen selector to ensure that the holder or a grid bar is not blocking the beam. Do not turn any knobs or push buttons. Contact lab personnel for help.

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